Schmid, Thomas E.; Dollinger, Günther; Beisker, Wolfgang; Hable, Volker; Greubel, Christoph; Auer, Susanne; Mittag, Anja; Tarnok, Attila; Friedl, Anna A.; Molls, Michael; Röper, Barbara
Document type:
Zeitschriftenartikel / Journal Article
Title:
Differences in the kinetics of γ-H2AX fluorescence decay after exposure to low and high LET radiation
Journal:
International Journal of Radiation Biology
Volume:
86
Issue:
8
Year:
2010
Pages from - to:
682-691
Language:
Englisch
Keywords:
H2AX ; carbon ions ; repair ; kinetics
Abstract:
Purpose: In order to obtain more insight into heavy ion tumour therapy, some features of the underlying molecular mechanisms controlling the cellular response to high linear energy transfer (LET) radiation are currently analysed. Materials and methods: We analysed the decay of the integrated fluorescence intensity of γ-H2AX (phosphorylated histone H2AX) which is thought to reflect the repair kinetics of radiation-induced DNA double-strand breaks (DSB) using Laser-Scanning-Cytometry. Asynchronous human HeLa cells were irradiated with a single dose of either 1.89 Gy of 55 MeV carbon ions or 5 Gy of 70 kV X-rays. Results: Measurements of the γ-H2AX-intensities from 15–60 min resulted in a 16 Conclusion: γ-H2AX loss kinetics follows a bi-exponential decline with two definite decay times independent of LET. The higher contribution of the slow component determined for carbon ion exposure is thought to reflect the increased amount of complex DSB induced by high LET radiation. «
Purpose: In order to obtain more insight into heavy ion tumour therapy, some features of the underlying molecular mechanisms controlling the cellular response to high linear energy transfer (LET) radiation are currently analysed. Materials and methods: We analysed the decay of the integrated fluorescence intensity of γ-H2AX (phosphorylated histone H2AX) which is thought to reflect the repair kinetics of radiation-induced DNA double-strand breaks (DSB) using Laser-Scanning-Cytometry. Asynchronous... »