Hauptner, Andreas; Krücken, Reiner; Greubel, Christoph; Hable, Volker; Dollinger, Günther; Drexler, Guido A.; Deutsch, M.; Löwe, R.; Friedl, Anna A.; Dietzel, Steffen; Strickfaden, Hilmar; Cremer, Thomas
Dokumenttyp:
Zeitschriftenartikel / Journal Article
Titel:
DNA-repair protein distribution along the tracks of energetic ions
Zeitschrift:
Radiation Protection Dosimetry
Jahrgang:
122
Heftnummer:
1-4
Jahr:
2006
Seiten von - bis:
147-149
Sprache:
Englisch
Stichwörter:
carbon ; ion ; lithium ; DNA ; DNA binding protein ; heavy ion, article ; cell damage ; cell nucleus ; chromatin ; controlled study ; HeLa cell ; human ; human cell ; immunofluorescence ; irradiation ; protein localization ; radiation injury ; chemical model ; chemical structure ; chemistry ; computer simulation ; DNA damage ; DNA repair ; linear energy transfer ; radiation dose ; radiation exposure ; radiation response, Chromatin ; Computer Simulation ; DNA ; DNA Damage ; DNA Repair ; DNA-Binding Proteins ; Dose-Response Relationship, Radiation ; Heavy Ions ; Hela Cells ; Humans ; Linear Energy Transfer ; Models, Chemical ; Models, Molecular ; Radiation Dosage «
carbon ; ion ; lithium ; DNA ; DNA binding protein ; heavy ion, article ; cell damage ; cell nucleus ; chromatin ; controlled study ; HeLa cell ; human ; human cell ; immunofluorescence ; irradiation ; protein localization ; radiation injury ; chemical model ; chemical structure ; chemistry ; computer simulation ; DNA damage ; DNA repair ; linear energy transfer ; radiation dose ; radiation exposure ; radiation response, Chromatin ; Computer Simulation ; DNA ; DNA Da... »
Abstract:
A simple model of homogenous chromatin distribution in HeLa-cell nuclei suggests that the track of an energetic ion hits 30 nm chromatin fibers with a mean distance of 0.55 μm. To test this assumption, living HeLa-cells were irradiated at the irradiation setup of the ion microprobe SNAKE using the ion beams provided by the Munich 14 MV tandem accelerator. After irradiation, the distribution of 53BP1 protein foci was studied by immunofluorescence. The observed 53BP1 distribution along the tracks of 29 MeV 7Li ions and 24 MeV 12C ions differed significantly from the expectations resulting from the simple chromatin model, suggesting that the biological track structure is determined by cell nuclear architecture with higher order organisation of chromatin. «
A simple model of homogenous chromatin distribution in HeLa-cell nuclei suggests that the track of an energetic ion hits 30 nm chromatin fibers with a mean distance of 0.55 μm. To test this assumption, living HeLa-cells were irradiated at the irradiation setup of the ion microprobe SNAKE using the ion beams provided by the Munich 14 MV tandem accelerator. After irradiation, the distribution of 53BP1 protein foci was studied by immunofluorescence. The observed 53BP1 distribution along the tracks... »