Hydrogen microscopy and analysis of DNA repair using focused high energy ion beams
Titel Sammlung:
17th International Conference on Ion Beam Analysis
Herausgeber Sammlung:
Boerma, Dirk O.; Climent-Font, Aurelio; Respaldiza, Miguel Angel; Ager, Francisco J.; García-López, Gastón
Zeitschrift:
Nuclear Instruments and Methods in Physics Research Section B
Jahrgang:
249
Heftnummer:
1-2
Konferenztitel:
International Conference on Ion Beam Analysis (17., 2005, Sevilla)
Tagungsort:
Sevilla, Spain
Jahr der Konferenz:
2005
Jahr:
2006
Seiten von - bis:
270-277
Sprache:
Englisch
Stichwörter:
Fluorescence ; Hydrogen ; Ion beams ; Microscopic examination ; Particle accelerators, High energy ion beams ; Hydrogen microscopy ; Ion energies ; Proton scattering, DNA
Abstract:
The ion microprobe SNAKE (Supraleitendes Nanoskop für Angewandte Kernphysikalische Experimente) at the Munich 14 MV tandem accelerator achieves beam focussing by a superconducting quadrupole doublet and can make use of a broad range of ions and ion energies, i.e. 4-28 MeV protons or up to 250 MeV gold ions. Due to these ion beams, SNAKE is particularly attractive for ion beam analyses in various fields. Here we describe two main applications of SNAKE. One is the unique possibility to perform three-dimensional hydrogen microscopy by elastic proton-proton scattering utilizing high energy proton beams. The high proton energies allow the analysis of samples with a thickness in the 100 μm range with micrometer resolution and a sensitivity better than 1 ppm. In a second application, SNAKE is used to analyse protein dynamics in cells by irradiating live cells with single focussed ions. Fluorescence from immunostained protein 53BP1 is used as biological track detector after irradiation of HeLa cells. It is used to examine the irradiated region in comparison with the targeted region. Observed patterns of fluorescence foci agree reasonably well with irradiation patterns, indicating an overall targeting accuracy of about 2 μm while the beam spot size is less than 0.5 μm in diameter. This performance shows successful adaptation of SNAKE for biological experiments where cells are targeted on a sub-cellular level by energetic ions. «
The ion microprobe SNAKE (Supraleitendes Nanoskop für Angewandte Kernphysikalische Experimente) at the Munich 14 MV tandem accelerator achieves beam focussing by a superconducting quadrupole doublet and can make use of a broad range of ions and ion energies, i.e. 4-28 MeV protons or up to 250 MeV gold ions. Due to these ion beams, SNAKE is particularly attractive for ion beam analyses in various fields. Here we describe two main applications of SNAKE. One is the unique possibility to perform thr... »